| Rank | Name | Team | Time | Venue |
| 1 |
MITCH LARKIN |
STPET |
53.26 |
Brisbane |
| 2 |
JACK GERRARD |
MVC |
53.49 |
Melbourne |
| 3 |
JOSHUA COLLETT |
KNXP |
54.84 |
Sydney |
| 4 |
TOMAS ELLIOTT |
RAVN |
54.89 |
Sydney |
| 5 |
LOUIS TOWNSEND |
RACKL |
55.00 |
Brisbane |
| 6 |
LIAM HUNTER |
CHAND |
55.24 |
Brisbane |
| 7 |
MATTHEW WILSON |
SOSC |
55.89 |
Brisbane |
| 8 |
LUKE BIBBY |
NBSC |
55.93 |
Sydney |
| 9 |
TOM JEFFRIES |
RACKL |
55.95 |
Brisbane |
| 10 |
ANDREW NEWLING |
RAVN |
56.36 |
Sydney |
| 11 |
ROBERT FRIEND |
YERPK |
56.60 |
Brisbane |
| 12 |
JACKSON ABEYA |
CHRCH |
56.63 |
Brisbane |
| 13 |
JOHANN STICKLAND |
PROP |
56.67 |
Melbourne |
| 14 |
NICHOLAS FINDEISEN |
FAS |
56.74 |
Melbourne |
| 15 |
SAMUEL WILLIAMSON |
FAS |
56.92 |
Melbourne |
| 16 |
NASH WILKES |
FOST |
56.99 |
Sydney |
| 17 |
CHARLIE HAWKE |
HUNT |
57.26 |
Sydney |
| 18 |
DANIEL CAVE |
MVC |
57.36 |
Melbourne |
| 19 |
EVAN CHEE |
NUN |
57.60 |
Melbourne |
| 20 |
RYAN BICKNELL |
NUN |
57.88 |
Melbourne |
| 21 |
ALEX MILLIGAN |
UWSC |
57.97 |
Perth |
| 22 |
KARL ALBERTYN |
KNXP |
58.05 |
Sydney |
| 23 |
BEN ARMBRUSTER |
STANT |
58.12 |
Brisbane |
| 24 |
LIAM LAIDLAW |
PROP |
58.20 |
Melbourne |
| 25 |
ALEKSANDR BELL |
UWSC |
58.26 |
Perth |
| 26 |
HAMISH MCDOUGALL |
SYP |
58.79 |
Melbourne |
| 27 |
TIM BELSTEAD |
HA |
59.10 |
Hobart |
| 28 |
YANNING ZHANG |
NUN |
59.11 |
Melbourne |
| 29 |
ADAM SELWOOD |
PROP |
59.14 |
Melbourne |
| 30 |
MATTHEW GEORGEVITS |
NBSC |
1:00.09 |
Sydney |
| 31 |
TYSON CADDY |
SYP |
1:00.10 |
Melbourne |
| 32 |
DAMIAN CULGAN |
NT |
1:00.88 |
Sydney |
| 33 |
DYLAN LOGAN |
GEE |
1:01.00 |
Melbourne |
| 34 |
LUKE GEBBIE |
MVC |
1:01.20 |
Melbourne |
| 35 |
JOSHUA HARGREAVES |
NUN |
1:02.62 |
Melbourne |
| 36 |
ETHAN JONES |
HC |
1:03.10 |
Hobart |
| 37 |
NATHAN TRAN |
NUN |
1:03.27 |
Melbourne |